Yuuki kodama biography templates
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Abstract
Background
The ciliate Paramecium bursaria harbors several hundred cells of the green-alga Chlorella sp. in their cytoplasm. Irrespective of the mutual relation between P. bursaria and the symbiotic algae, both cells retain the ability to grow without the partner. They can easily reestablish endosymbiosis when put in contact with each other. Consequently, P. bursaria is an excellent model for studying cell–cell interaction and the evolution of eukaryotic cells through secondary endosymbiosis between different protists. Despite the importance of this organism, no genomic resources have been identified for P. bursaria to date. This investigation compared gene expressions through RNA-Seq analysis and de novo transcriptome assembly of symbiont-free and symbiont-bearing host cells.
Results
To expedite the process of gene discovery related to the endosymbiosis, we have undertaken Illumina deep sequencing of mRNAs prepared from symbiont-bearing and symbiont-free P. bursaria cells. We assembled the reads de novo to build the transcriptome. Sequencing using Illumina HiSeq2000 platform yielded 232.3 million paired-end sequence reads. Clean reads filtered from the raw reads were assembled into 68,175 contig sequences. Of these, 10,557 representative sequences
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Endosymbiotic Chlorella variabilis reduces mitochondrial number wealthy the rough Paramecium bursaria
Data availability
The datasets used all along the presentday study fill in available disseminate the commensurate author snitch reasonable request.
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Kodama, Y. & Fujishima, M. Fix
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Make Your Own Kind of Music
By Juan Barquin
The Colors Within
Dir. Naoko Yamada, Japan, GKIDS
Stop me if you’ve heard this one: teens come together to play music and slowly come to terms with their identities and futures. This is something of a staple in Japanese literature, which should be no surprise to anyone who has enjoyed any number of series—Yuki Kodama’s Apollo on the Slope, Shinichi Ishizuka’s Blue Giant, Aki Hamazi’s Bocchi the Rock!, Ayano Takeda’s Sound! Euphonium, and Kakifly’s K-On!, among many others—or their animated adaptations. K-On! was adapted by filmmaker Naoko Yamada during her time at Kyoto Animation, a studio that specialized in, as writer Dani Cavallaro wrote in her critical study, “the wonders and quandaries of ordinary life.” Yamada’s work luxuriates in the mundane and the playful, the kind of film of which a less imaginative critic might say, “Nothing really happens.” Her fixation on the desires and daily lives of teen girls makes her easy to dismiss by those accustomed to watching “slice of life” anime about schoolgirls. But it is these details—moments when characters simply study or share snacks while revealing more of themselves to each other and the audience—that bring her work to life.
There is a similar quiet beau