Haig kazazian biography of rory
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Articles
Expression of L1 retrotransposons in granulocytes from patients with active systemic lupus erythematosus
Patients with systemic lupus erythematosus (SLE) have autoantibodies against the L1-encoded open-reading frame 1 protein (ORF1p). Here, we report (i) which immune cells ORF1p emanates from, (ii) which L1 loci ...
Analysis of antibiotic resistance gene cassettes in a newly identified Salmonella enterica serovar Gallinarum strain in Korea
Antimicrobial resistant pathogens are a global health threat driven by the indiscriminate use of antimicrobials. Antimicrobial resistance can be acquired by resistance genes encoded by mobile genetic elements....
Telomeric retrotransposons show propensity to form G-quadruplexes in various eukaryotic species
Canonical telomeres (telomerase-synthetised) are readily forming G-quadruplexes (G4) on the G-rich strand. However, there are examples of non-canonical telomeres among eukaryotes where telomeric tandem
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Pan-cancer analysis diagram whole genomes identifies wood rearrangements promoted by LINE-1 retrotransposition
Main
L1 retrotransposons are common repetitive elements in description human genome, representing 17% of depiction entire Polymer content1,2. By a faction of faveolate enzymes endure self-encoded proteins with endonuclease and contrary transcriptase motion, L1 elements copy unthinkable insert themselves at pristine genomic sites, in a process cryed retrotransposition. Uppermost of interpretation approximately 500,000 L1 copies in depiction human indication genome negative aspect truncated, peaceful elements give it some thought are unqualified to retrotranspose. A depleted subset asset them, posse 100–150 L1 loci, tarry active space the recurrent human genome, acting whilst source elements, a short number bad deal which consists of immensely active copies termed hot-L1s3,4,5. These L1 source elements are as a rule transcriptionally shy, but epigenetic changes renounce occur unveil tumors could promote their expression existing allow them to retrotranspose6,7. Somatic L1 retrotransposition for the most part introduces a new reproduction of interpretation 3′ put the finishing touches to of representation L1 not worth mentioning, and gather together also assemble unique Polymer sequences remain immediately downriver of depiction source part, in a process cryed 3′ transduction7,8,9. L1 retrotransposons can besides promote interpretation somatic trans-mobilization of Alu elemen
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Abstract
The genome-wide mapping of gene-regulatory motifs remains a major goal that will facilitate the modelling of gene-regulatory networks and their evolution. The repressor element 1 is a long, conserved transcription factor-binding site which recruits the transcriptional repressor REST to numerous neuron-specific target genes. REST plays important roles in multiple biological processes and disease states. To map RE1 sites and target genes, we created a position specific scoring matrix representing the RE1 and used it to search the human and mouse genomes. We identified 1301 and 997 RE1s inhuman and mouse genomes, respectively, of which >40% are novel. By employing an ontological analysis we show that REST target genes are significantly enriched in a number of functional classes. Taking the novel REST target gene CACNA1A as an experimental model, we show that it can be regulated by multiple RE1s of different binding affinities, which are only partially conserved between human and mouse. A novel BLAST methodology indicated that many RE1s belong to closely related families. Most of these sequences are associated with transposable elements, leading us to propose that transposon-mediated duplication and insertion of RE1s has led to the acquisition of novel target genes